Description and DNA barcoding of Tipula (Pterelachisus) recondita sp. n. from the Palaearctic region (Diptera, Tipulidae)

Collection: Pensoft Publishers

Keywords: Crane flies · Topics: Diptera species taxonomy and behavior, Bryophyte Studies and Records, Hemiptera Insect Studies

Tipula (Pterelachisus) recondita Pilipenko & Salmela, sp. n. is described. The new species is collected from two localities: Finland, Kittilä (North boreal ecoregion) and Russia, Primorski kray (Zone of temperate broadleaf and mixed forests). Although variation in the structure of male hypopygium between the Finnish and Russian populations is observed, DNA barcode sequences differ only by three nucleotides (0.2 % K2P distance), supporting presence of one widespread species. K2P minimum distances between the new species and 17 other species of the subgenus range from 5.3 to 15.8 % (mean 8.8 %). The new species is forest-dwelling, known from an old-growth herb-rich forest (Finland) and Quercus mongolica forest (Russia). The new species is perhaps closest to T. (P.) imitator Alexander and in lesser extent to T. (P.) pauli Mannheims; the inner gonostylus of both species are illustrated.

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