Phylogeny of the Harnischia generic complex (Diptera, Chironomidae) inferred from twenty whole mitogenomes
This study presents the first comprehensive mitochondrial genome analysis of three Chironomidae genera, including sequencing, assembling, and annotating mitogenomes from two Paracladopelma species, one Parachironomus species and one Harnischia species. These newly characterized mitogenomes were subjected to comparative genomic analysis alongside twenty previously published Chironomidae mitogenomes, enabling robust phylogenetic reconstruction within the Harnischia generic complex. The Ka/Ks ratio analysis reveals that most protein-coding genes (PCGs) have undergone purifying selection, with ND6, ATP8, and ND5 exhibiting higher ω values and thus greater evolutionary flexibility. Phylogenetic analyses using Bayesian inference and Maximum likelihood methods demonstrate Robackia as a basal group. This study expands the available mitogenomic database and provides a robust foundation for future phylogenetic analyses of the Harnischia generic complex.
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