Works on Environmental DNA in Biodiversity Studies
Published research works tagged with the topic “Environmental DNA in Biodiversity Studies”.
276 works
Showing 151 to 200 of 276 works
Comparison of three strategies for local bioassessments in streams using environmental DNA and RNA metabarcoding of mac…
Environmental DNA (eDNA) metabarcoding of streams can deliver information on biological communities with minimally invasive efforts and at low costs. However, the captured eDNA ma…
Gradient patterns in the Tatra Mountain lakes revisited with invertebrate DNA metabarcoding
Monitoring changes in freshwater fauna requires timely, efficient, and comprehensive data collection, needs that are increasingly met by molecular methods. This is especially impo…
Influence of homogenization methods on lichen species detection from environmental DNA metabarcoding
Environmental DNA (eDNA) techniques are increasingly employed in biodiversity monitoring of aquatic and terrestrial animals, plants, and fungi, holding great potential to revoluti…
Proficiency testing and cross-laboratory method comparison to support standardisation of diatom DNA metabarcoding for f…
DNA metabarcoding of benthic diatoms has been successfully applied for biomonitoring at the national scale and can now be considered technically ready for routine application. How…
Application of a dual-locus metabarcoding approach for a more comprehensive account of cattle dietary items in a semi-a…
Increasing livestock densities and more severe drought events challenge sustainable management in South Africa’s semi-arid savannas. Effective mitigation strategies require accura…
Autonomous samplers and environmental DNA metabarcoding: sampling day and primer choice have greatest impact on fish de…
Unprecedented rates of biodiversity loss and ecosystem function necessitate the use of rapid, efficacious, and cost-effective biomonitoring tools. The combination of autonomous sa…
Impact of a coastal protection measure on sandy-beach meiofauna at Ahrenshoop (Baltic Sea, Germany): results from metab…
Sand nourishment is a common and often applied coastal protection measure along the beaches of the German Baltic Sea coast. At the tourist beach of Ahrenshoop, large-scale sand no…
Insights into the ecological impact of trout introduction in an oligotrophic lake using sedimentary environmental DNA
Introduced trout can induce trophic cascades, however, a lack of pre-introduction data limits knowledge on their impact in many lakes. Traditional paleolimnological approaches hav…
Exploring benthic diatom diversity in the West Antarctic Peninsula: insights from a morphological and molecular approa…
Polar regions are among the most extreme habitats on Earth. However, diatom biodiversity in those regions is much more extensive and ecologically diverse than previously thought. …
Fish diversity assessed by eDNA detection methods in the Rioni River
Due to anthropogenic influences, habitat degradation and a continuous loss of biodiversity in freshwater ecosystems are occurring on a large scale, while these ecosystems constitu…
Unravelling bird nest arthropod community structure using metabarcoding
Bird nests are fascinating microcosms harboring a wide range of arthropods parasitizing the nesting birds or feeding on prey remains, feces, and the nest material. Studies of thes…
Ecological signals of arctic plant-microbe associations are consistent across eDNA and vegetation surveys
Understanding how different taxa respond to abiotic characteristics of the environment is of key interest for understanding the assembly of communities. Yet, whether eDNA data wil…
DNA metabarcoding data from faecal samples of the lesser (Myotis blythii) and the greater (Myotis myotis) mouse-eared …
A comprehensive understanding of trophic interactions in terrestrial ecosystems is crucial for ecological research and conservation. Recent advances in non-invasive methods, such …
Direct PCR meets high-throughput sequencing – metabarcoding of chironomid communities without DNA extraction
Aquatic emergent insect communities form an important link between aquatic and terrestrial ecosystems, yet studying them is costly and time-consuming as they are usually diverse a…
Pollen metabarcoding of museum specimens and recently collected bumblebees (Bombus) indicates foraging shifts
Landscape changes, over time, lead to changes of floral resources available to pollinators, which in turn may result in the disappearance of ecologically specialized species. Here…
Development of two new sets of PCR primers for eDNA metabarcoding of brittle stars (Echinodermata, Ophiuroidea)
Brittle stars (class Ophiuroidea) are marine invertebrates comprising approximately 2,100 extant species, and are considered to constitute the most diverse taxon of the phylum Ech…
Recommendations for the preservation of environmental samples in diatom metabarcoding studies
Implementation of DNA metabarcoding for diatoms for environmental monitoring is now moving from a research to an operational phase, requiring rigorous guidelines and standards. In…
Metabarcoding as an effective complement of microscopic studies in revealing the composition of the diatom community –…
Diatoms are valuable bioindicators and their traditional classification and identification are mainly based on the morphological characteristics of their frustules. However, in re…
The relationship between eDNA density distribution and current fields around an artificial reef in the waters of Tatey…
Monitoring of artificial reefs (ARs) has been conducted through such methods as visual censuses, surveys using fishing gear, and echo sounder. These methods have disadvantages: vi…
Monitoring harmful microalgal species and their appearance in Tokyo Bay, Japan, using metabarcoding
During the recent decade, high-throughput sequencing (HTS) techniques, in particular, DNA metabarcoding, have facilitated increased detection of biodiversity, including harmful al…
COI amplicon sequence data of environmental DNA collected from the Bronx River Estuary, New York City
In this data paper, we describe environmental DNA (eDNA) cytochrome c oxidase (COI) amplicon sequence data from New York City’s Bronx River Estuary. As urban systems continue to e…
The bacterial hitchhiker’s guide to COI: Universal primer-based COI capture probes fail to exclude bacterial DNA, but …
Environmental DNA (eDNA) metabarcoding from water samples has, in recent years, shown great promise for biodiversity monitoring. However, universal primers targeting the cytochrom…
18S rDNA amplicon sequence data (V1–V3) of the Palmyra Atoll National Wildlife Refuge, Central Pacific
To address the global biodiversity crisis, standardized data that are rapidly obtainable through minimally invasive means are needed for documenting change and informing conservat…
Metagenomic insights to the functional potential of sediment microbial communities in freshwater lakes
Molecular-based techniques offer considerable potential to provide new insights into the impact of anthropogenic stressors on lake ecosystems. Microbial communities are involved i…
Annual dynamics of eukaryotic and bacterial communities revealed by 18S and 16S rRNA metabarcoding in the coastal ecos…
Sagami Bay, Japan is influenced by both the warm Kuroshio Current and the cold Oyashio Current and rich nutrients are supplied from multiple river sources and the deep-sea, formin…
Shifts in DNA yield and biological community composition in stored sediment: implications for paleogenomic studies
Lake sediments hold a wealth of information from past environments that is highly valuable for paleolimnological reconstructions. These studies increasingly apply modern molecular…
Comparing species detection success between molecular markers in DNA metabarcoding of coastal macroinvertebrates
DNA metabarcoding has great potential to improve marine biomonitoring programs by providing a rapid and accurate assessment of species composition in zoobenthic communities. Howev…
Using DNA metabarcoding to decipher the diet plant component of mammals from the Eastern Mediterranean region
Longevity of species populations depends largely on interactions among animals and plants in an ecosystem. Predation and seed dispersal are among the most important interactions n…
18S rRNA amplicon sequence data (V1–V3) of the Bronx river estuary, New York
Characterising and monitoring biological diversity to foster sustainable ecosystems is highly recommended as urban centres rapidly expand. However, much of New York City’s biodive…
Beyond fish eDNA metabarcoding: Field replicates disproportionately improve the detection of stream associated vertebra…
Fast, reliable, and comprehensive biodiversity monitoring data are needed for environmental decision making and management. Recent work on fish environmental DNA (eDNA) metabarcod…
Potential for cross-contamination of diatom DNA samples when using toothbrushes
The use of toothbrushes and similar devices for sampling diatoms from hard surfaces is a well-established approach. Toothbrushes are routinely cleaned and reused when sampling for…
Methodological considerations for monitoring soil/litter arthropods in tropical rainforests using DNA metabarcoding, wi…
Robust data to refute or support claims of global insect decline are currently lacking, particularly for the soil fauna in the tropics. DNA metabarcoding represents a powerful app…
Soil stabilisation for DNA metabarcoding of plants and fungi. Implications for sampling at remote locations or via thir…
Storage of soil samples prior to metagenomic analysis presents a problem. If field sites are remote or if samples are collected by third parties, transport to analytical laborator…
The mitochondrial genomes of 11 aquatic macroinvertebrate species from Cyprus
Aquatic macroinvertebrates are often identified, based on morphology, but molecular approaches like DNA barcoding, metabarcoding and metagenomics are increasingly used for species…
Metabarcoding of soil nematodes: the importance of taxonomic coverage and availability of reference sequences in choosi…
For many organisms, there is agreement on the specific genomic region used for developing barcode markers. With nematodes, however, it has been found that the COI region designate…
DNA metabarcoding of Neotropical ichthyoplankton: Enabling high accuracy with lower cost
Knowledge of ichthyoplankton dynamics is extremely important for conservation management as it can provide information about preferential spawning sites, reproductive period, migr…
Development of a new set of PCR primers for eDNA metabarcoding decapod crustaceans
The Decapoda is one of the largest orders within the class Malacostraca, comprising approximately 14,000 extant species and including many commercially important species. For biod…
Optimising the detection of marine taxonomic richness using environmental DNA metabarcoding: the effects of filter mate…
The analysis of environmental DNA (eDNA) using metabarcoding has increased in use as a method for tracking biodiversity of ecosystems. Little is known about eDNA in marine human-m…
Choice of DNA extraction method affects DNA metabarcoding of unsorted invertebrate bulk samples
Characterisation of freshwater benthic biodiversity using DNA metabarcoding may allow more cost-effective environmental assessments than the current morphological-based assessment…
Characterising planktonic dinoflagellate diversity in Singapore using DNA metabarcoding
Dinoflagellates are traditionally identified morphologically using microscopy, which is a time-consuming and labour-intensive process. Hence, we explored DNA metabarcoding using h…
Estimating fish alpha- and beta-diversity along a small stream with environmental DNA metabarcoding
Environmental DNA (eDNA) metabarcoding has been increasingly applied to biodiversity surveys in stream ecosystems. In stream networks, the accuracy of eDNA-based biodiversity asse…
Dropping Hints: Estimating the diets of livestock in rangelands using DNA metabarcoding of faeces
The introduction of domesticated animals into new environments can lead to considerable ecological disruption, and it can be difficult to predict their impact on the new ecosystem…
New species of the pseudoscorpion genus Pararoncus (Pseudoscorpiones, Syarinidae) from Korea
Two new species of the pseudoscorpion genus Pararoncus Chamberlin, 1938 are described from Korea. Detailed descriptions, habitus pictures, and illustrations of Pararoncus leonardi…
Phylogeography, species delimitation and population structure of a Western Australian short-range endemic mite harvestm…
The mite harvestmen of the genus Karripurcellia Giribet, 2003 are endemic to the tall, wet eucalypt forests of south-western Western Australia, a region known as a hotspot for bio…
First report of Cloeon vanharteni Gattolliat & Sartori, 2008 (Baetidae, Ephemeroptera) in the Maghreb
Cloeon vanharteni Gattolliat & Sartori, 2008 was newly discovered in the framework of our study of Ephemeroptera in the Draa basin, located in the southern region of the High Atla…
New species of Labiobaetis Novikova & Kluge from New Guinea (Ephemeroptera, Baetidae): a never-ending story of diversity
Investigations of material collected partly in 1999 and mainly between 2006 and 2016 in New Guinea, mostly along the high, central mountain chain of the island, further increased …
Rediscovery of Stactobia eatoniella McLachlan, 1880 (Trichoptera, Hydroptilidae) in Switzerland after more than seventy…
A specimen of Stactobia eatoniella, a hygropetric species of micro-caddisfly considered extinct in Switzerland, was discovered in the Morge in Valais in March 2020 during routine …
A new species of Cincticostella (Ephemeroptera, Ephemerellidae) from China
Cincticostella tornata, a new species of Ephemerellidae, is described from China. It belongs to the insolta-species group and is closely related to C. femorata. It is hitherto onl…
Exploring the dietary niche of Atlantolacerta andreanskyi (Lacertidae) using DNA metabarcoding
Determining the dietary niche is an essential part of any conservation strategy, and for modeling the community responses to climate change. DNA metabarcoding methods are revoluti…
Oomycete-specific ITS primers for identification and metabarcoding
Microbial metabarcoding studies using high throughput sequencing technologies generate unprecedented amounts of DNA sequence data and make it possible to determine not only the co…