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Showing 2301 to 2350 of 6881 works
Stemflow for detecting mammalian environmental DNA: a case study in a zoo
As mammalian diversity declines, effective biodiversity monitoring tools are urgently needed. To address this issue, we assessed the detectability of mammalian environmental DNA (…
Live and dead fish shed different amounts of intact cells: Implications for advancing environmental DNA methodologies
Environmental DNA (eDNA) analysis has significantly contributed to biodiversity monitoring due to its sampling simplicity and inherent high sensitivity. While the technique has ma…
Guidance and best practices for species identification using eDNA metabarcoding – When do you call a cod a cod?
With the rapid uptake of environmental DNA (eDNA) metabarcoding for a wide range of conservation and management uses, there is a growing need for guidance and best practices for s…
Navigating the European aquatic eDNA landscape: Opportunities for metadata standardisation and data mobilisation
Environmental DNA (eDNA) has emerged as a transformative tool for monitoring aquatic biodiversity, offering a non-invasive and highly sensitive approach to detecting organisms acr…
A 12S rDNA barcode reference library for Neotropical electric fishes (Teleostei, Gymnotiformes)
DNA barcodes are instrumental for identifying species from tissues and environmental samples. Metabarcoding-based assessments of biodiversity depend on taxonomically accurate barc…
A 178 bp diagnostic D-loop fragment (OvisCR) to discriminate domestic sheep (Ovis aries) and European mouflon (Ovis mus…
Accurate species identification is essential in metabarcoding diet studies, notably to distinguish domestic from wild species, as they have markedly different ecological and socio…
DNA barcoding reference dataset revealed overlooked diversity and clarified biogeography of fishes in the largest Europ…
This study represents the first thorough genetic assessment of freshwater fishes from the Volga River Basin – the longest (3,690 km) river in Europe. DNA barcode sequences (COI) w…
Modular universal rbcL primers for (meta)barcoding: a systematic case study clarifying the identity of the marine macro…
The geniculate coralline algal genus Corallina L. (Rhodophyta, Corallinales) presents longstanding challenges in taxonomy and nomenclature. Recent studies have uncovered previousl…
Welcome to Hotel Hymenoptera: monitoring cavity-nesting bee and wasp distribution and their trophic interactions using …
Essential ecosystem services are provided by many interactions, including plant-pollinator, predator-prey, and host-parasitoid. These services support food and natural systems thr…
Applying flukeprint environmental DNA to detect skin-associated bacteria from free-ranging West Coast Transient killer …
Globally, vessel strikes, entanglements, and ocean pollution negatively impact cetaceans by compromising skin integrity and health through direct injury or disease and by increasi…
Baseline studies on meiofauna in the Baltic Sea before bottom-trawl fisheries exclusion II. A comparative study using m…
Understanding how meiofaunal communities respond to spatial protection measures is essential for evaluating the effectiveness of marine conservation strategies, particularly in he…
Constraining lake ecology via metagenomics, metatranscriptomics, and amplicon sequencing
Lake ecosystems are hotspots for important ecosystem functions, yet linking their microbiome to physicochemical parameters remains a challenge. Here, we compare 16S rRNA gene-base…
A DNA metabarcoding workflow for the detection of terrestrial gastropods from ingested DNA
DNA metabarcoding is a powerful approach for assessing biodiversity and unravelling trophic interactions, particularly in the context of biological invasions where introduced pred…
Invasive species monitoring based on eDNA multiplex PCR sequencing
Invasive species pose a significant threat to global biodiversity and the stability of ecosystems. Although environmental DNA (eDNA)-based quantitative PCR is considered effective…
A comparative analysis of hybridisation capture and PCR-based eDNA metabarcoding for monitoring bryophytes in riparian …
Despite their ecological importance, mosses remain under-represented in ecological studies due to their challenging detection in field surveys and morphological identification, ex…
From short to long reads: enhanced protist diversity profiling via Nanopore metabarcoding
In the last decades, environmental metabarcoding has revolutionised biodiversity research, particularly for microbial organisms such as protists, enabling large-scale assessments …
Evaluating the potential of DNA metabarcoding for ecological status assessment under the Water Framework Directive: A c…
Routine biomonitoring under the Water Framework Directive (WFD) follows a conventional methodology primarily based on the morphological identification of taxa within the five biol…
From Asia through America to Europe: Molecular and morphological evidence for a non-indigenous diatom species, Discoste…
This study presents the first occurrences of the diatom species Discostella asterocostata in Europe’s second largest river, the Danube River and the earliest occurrence in Europea…
Mind the gap: DNA barcoding on Pachygrapsus crabs
DNA barcode databases for marine invertebrates faces significant challenges, with several species revealing taxonomic issues like misidentification, ambiguity or shared barcodes. …
Markers or metagenomes: sequencing marine eukaryotic DNA for better biodiversity surveys
Marker gene sequencing (“metabarcoding”) is the primary sequencing approach currently used for molecular biodiversity surveys, but this approach is taxonomically limited and hampe…
DNA metabarcoding reveals the influence of land cover and farming on the dietary composition of a spider-specialist bat
Human-driven landscape transformations are the primary factors driving biodiversity changes in the present century. For insectivorous bats, land-use modifications and intensificat…
Microbial biodiversity and metabolic functioning in sediments of coastal dune lakes on a remote island
Molecular-based techniques provide the potential for novel insights into the functioning of ecosystems, especially those that are globally rare such as coastal dune lakes. In the …
Case study of non-lethal sampling for plant-pollinator networks via barcoding and metabarcoding on bumble bees in Germa…
1. Insect decline is threatening ecosystem stability, making information about foraging preferences of pollinators a vital piece of information to acquire. A powerful emerging too…
An annotated reference library for supporting DNA metabarcoding analysis of aquatic macroinvertebrates in French freshw…
Freshwater ecosystems are increasingly threatened by human activities, leading to biodiversity loss and ecosystem degradation. Effective biodiversity monitoring, particularly thro…
Comparing morphological and DNA-based bioassessment methodologies for macroinvertebrates in Neotropical streams: a case…
As unimpacted freshwater environments decline worldwide, developing effective and accurate biomonitoring approaches is critical for detecting ecosystem deterioration and facilitat…
What is eDNA method standardisation and why do we need it?
The rapid advancement of environmental DNA (eDNA) science in the past two decades has inspired a concomitant growth in the development of eDNA sampling and analytical methods. How…
Technology Readiness Level of biodiversity monitoring with molecular methods – where are we on the road to routine impl…
Human activities are causing rapid biodiversity loss across ecosystems, affecting human well-being and crucial ecosystem services. Traditional biodiversity monitoring tools cannot…
Inter-laboratory ring test for environmental DNA extraction protocols: implications for marine megafauna detection usin…
The comparability of methods applied to environmental DNA (eDNA) samples across laboratories remains a significant challenge for biodiversity monitoring on a global scale. Perform…
The MIEM guidelines: Minimum information for reporting of environmental metabarcoding data
Environmental DNA (eDNA) and RNA (eRNA) metabarcoding has become a popular tool for assessing biodiversity from environmental samples, but inconsistent documentation of methods, d…
Assessing the impact of tidal changes on fish environmental DNA metabarcoding in temperate and tropical coastal regions…
Environmental DNA (eDNA) analysis is a valuable tool for detecting species presence in coastal environments. However, its effectiveness can be influenced by factors such as diffus…
Homogenization of insect bulk samples yields more comprehensive yet comparable biodiversity data than non-destructive l…
The rapid advancement of molecular biodiversity monitoring tools, particularly DNA metabarcoding, has improved specimen identification in bulk samples, such as those from Malaise …
Comparison of the biodiversity of epiphytic diatoms in the Euphrates-Tigris rivers using morphological and metabarcodin…
The Euphrates-Tigris catchment area is one of the major drainage basins in the Middle East but climate change, overuse of waters and subsequent salinization has brought this regio…
NAMERS: a purpose-built reference DNA sequence database to support applied eDNA metabarcoding
Applied eDNA metabarcoding is increasingly being considered as a tool to inform management decisions, regulations, or policy development. Because these downstream considerations a…
A morphological and high throughput sequencing workflow to identify Australian ants (Hymenoptera, Formicidae): a new to…
Ants are often the most ubiquitous and ecologically influential components of terrestrial systems, exhibiting an exceptionally high level of endemic diversity. Simultaneously, exo…
Benchmarking the discrimination power of commonly used markers and amplicons in marine fish (e)DNA (meta)barcoding
Environmental DNA (eDNA) metabarcoding is revolutionising the study of aquatic ecosystems, enabling high-throughput analysis of biodiversity with minimal disturbance. Despite its …
The ASV Registry: a place for ASVs to be
Despite the effectiveness of DNA metabarcoding for gaining insights into biodiversity and environmental species composition, a centralised management and storage option including …
Centering accessibility, increasing capacity, and fostering innovation in the development of international eDNA standar…
Environmental DNA (eDNA) includes a set of rapidly emerging technologies that have the potential to support environmental monitoring and biodiversity conservation through novel, n…
Hidden in the blow - a matrix to characterise cetaceans’ respiratory microbiome: short-finned pilot whale as case study
Cetaceans are key sentinel species of marine ecosystems and ocean health, being a strategic taxonomic group that evaluates the well-being of aquatic habitats and detects harmful e…
Influence of storage time on the stability of diatom assemblages using DNA from riverine biofilm samples
DNA sequencing of diatom assemblages from biofilms has already been used to assess the ecological status of freshwater in the UK. However, recent work using DNA data from these bi…
Optimising eDNA analysis for urban otter monitoring: seasonal patterns, detection strategies and prey availability
Significant advancements in environmental DNA (eDNA) analysis technology have led to its widespread adoption for species monitoring. However, the low DNA concentration in eDNA oft…
Optimising species detection probability and sampling effort in lake fish eDNA surveys
Environmental DNA (eDNA) metabarcoding is transforming biodiversity monitoring in aquatic environments. Such an approach has been developed and deployed for monitoring lake fish c…
Improving understanding of nematode communities in agricultural settings: a comparison of mitometagenomics and morpholo…
Florida’s strawberry production provides significant economic benefit for the State; however, plant-parasitic nematodes (PPNs) pose a significant barrier to production. A better u…
Net type, tow duration and day/night sampling effects on the composition of marine zooplankton derived from metabarcodi…
DNA metabarcoding requires only a single DNA fragment to detect a species in mixed zooplankton samples, compared to morphology-based methods that rely on the presence of intact sp…
Nanopore duplex sequencing as an alternative to Illumina MiSeq sequencing for eDNA-based biomonitoring of coastal aquac…
Oxford Nanopore Technologies has recently launched a duplex sequencing strategy and announced an improved error rate which is in a similar order of magnitude as Illumina sequencin…
Simple approaches for evaluation of OTU quality based on dissimilarity arrays
An accurate and complete taxonomic description of the diversity present in an environmental sample is out of reach at this time. Instead, metabarcoding is used today and it is exp…
Assessing the diversity of nematodes in the Store Mosse National Park (Sweden) using metabarcoding
The Store Mosse National Park in the south of Sweden was surveyed for nematode diversity and distribution using DNA metabarcoding. Fifty samples were collected from five vegetatio…
Halamphora taxa in Hungarian soda pans and shallow soda lakes detected via metabarcoding and microscopic analyses
The research presented investigates whether DNA-based metabarcoding can replace the morphology-based identification of diatom taxa in the ecological status assessments of aquatic …
Abundance estimation with DNA metabarcoding – recent advancements for terrestrial arthropods
Biodiversity is declining at alarming rates worldwide and large-scale monitoring is urgently needed to understand changes and their drivers. While classical taxonomic identificati…
Natrix2 – Improved amplicon workflow with novel Oxford Nanopore Technologies support and enhancements in clustering, c…
Sequencing of amplified DNA is the first step towards the generation of Amplicon Sequence Variants (ASVs) or Operational Taxonomic Units (OTUs) for biodiversity assessment and com…
Evaluating five primer pairs for environmental DNA metabarcoding of Central European fish species based on mock commun…
Environmental DNA (eDNA) metabarcoding has become a powerful tool for examining fish communities. Prior to the introduction of eDNA-based assessments into regulatory monitoring co…
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